βcgrp  (GL Biochem)

Journal: Genes and Immunity

Article Title: αCGRP deficiency aggravates pulmonary fibrosis by activating the PPARγ signaling pathway

doi: 10.1038/s41435-023-00206-x

Figure Lengend Snippet: A , B The expression of βCGRP, BAX, PPARɤ, and TGFβ was higher in BLM and Calca +/− rats compared to WT group (_400). There was abnormal expression of CD3, CD68, βCGRP, BAX, PPARɤ and TGFβ in the BLM group and Calca +/− group. The TUNEL staining showed there were more positive alveolar epithelial cells in BLM group and Calca +/− group than in WT control group (_200). Predominant CD68+CD206+M2 subtype differentiation was found in both Calca +/− rats and BLM rats by immunofluorescence staining (In C , green represents CD68+ and red represents iNOS. In D , green represents CD68+ and red represents CD206+)(_200).

Article Snippet: Antigen retrieval was performed by cooking tissue sections for 30 min in Tris-EDTA buffer and applying the following primary antibodies: CD68 + (ready-to-use, Maixin, Fuzhou, China), CD3 + (1:1000, Santa Cruz, California, USA), TGFβ1 (1:800, Bioworld, Louis Park, MN, USA), βCGRP (1:800, ABclonal, Wuhan, China), αCGRP (1:800, ABclonal, Wuhan, China), BAX (1:500, proteintech, Chicago, USA), and PPAR-ɤ (1:800, Bioss, Beijing, China) at 4 °C overnight.

Techniques: Expressing, TUNEL Assay, Staining, Control, Immunofluorescence

Journal: PLoS ONE

Article Title: Calcitonin Gene-Related Peptide Regulates Type IV Hypersensitivity through Dendritic Cell Functions

doi: 10.1371/journal.pone.0086367

Figure Lengend Snippet: (A) The percentage of CD11c + BMDCs was analyzed by FACS. (B) BMDCs were incubated with or without CGRP in the presence of LPS stimulation. The cellular cAMP levels were determined after the indicated time periods. Values are presented as mean ± SD (n = 3). (C) The concentrations of each cytokine were determined by ELISA after 24 h stimulation with LPS. Values are presented as mean ± SD (n = 3). * P <0.05, ** P <0.01 VS. LPS only. (D) The concentrations of each cytokine were determined by ELISA after 24 h stimulation with LPS. Values are presented as mean ± SD (n = 3). * P <0.05, ** P <0.01. (E) The mRNA expression was determined by real-time PCR after 6 h stimulation with LPS. Values are presented as mean ± SD (n = 3). * P <0.05, ** P <0.01 vs. LPS only. α: αCGRP (1 nM), β: βCGRP (1 nM), dbc: db-cAMP (100 µM), 6b: 6-bnz-cAMP (100 µM), and 8C: 8-CPT-cAMP (100 µM) were added at the start of stimulation.

Article Snippet: LPS, PKA inhibitor H89, and phosphodiesterase inhibitor IBMX (3-isobtyl-1-methylxanthine) were purchased from Sigma Aldrich (St. Louis, MO). αCGRP was purchased from Peptide Institute (Osaka, Japan), and βCGRP was purchased from Phoenix Pharmaceuticals, Inc. (Burlingame, CA).

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Expressing, Real-time Polymerase Chain Reaction

Journal: PLoS ONE

Article Title: Calcitonin Gene-Related Peptide Regulates Type IV Hypersensitivity through Dendritic Cell Functions

doi: 10.1371/journal.pone.0086367

Figure Lengend Snippet: (A) The concentrations of each cytokine were determined by ELISA after 24 h stimulation with OVA and anti-CD40 mAb. Values are presented as means ± SD (n = 3). ** P <0.01 VS. OVA+anti-CD40 mAb. (B) The mRNA expression was determined by real-time PCR after 6 h stimulation with OVA and anti-CD40 mAb. Values are presented as mean ± SD (n = 3). * P <0.05, ** P <0.01 vs. OVA+anti-CD40 mAb. (C) BMDCs and DO 11.10 Th0 cells were co-cultured in the presence of 200 µg/ml OVA for 72 h. IFN-γ production was detected by FACS after intracellular staining. Data are represented of three independent experiments. α: αCGRP (1 nM), β: βCGRP (1 nM), dbc: db-cAMP (100 µM), 6b: 6-bnz-cAMP (100 µM), and 8C: 8-CPT-cAMP (100 µM) were added from start point of stimulation.

Article Snippet: LPS, PKA inhibitor H89, and phosphodiesterase inhibitor IBMX (3-isobtyl-1-methylxanthine) were purchased from Sigma Aldrich (St. Louis, MO). αCGRP was purchased from Peptide Institute (Osaka, Japan), and βCGRP was purchased from Phoenix Pharmaceuticals, Inc. (Burlingame, CA).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Real-time Polymerase Chain Reaction, Cell Culture, Staining